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Fig. 3
Overexpression of DYRK1A induces nanos1-marked PGCs deficiency in early zebrafish embryo. Analysis of localization and strength of nanos1 positive cells in DYRK1A overexpressed embryos by WISH at 50% Epiboly stage (a), 8-somite stage (b) and Prim-5 stage (c). Histogram representing the relative expression detected by qPCR in GFP injected and DYRK1A overexpressed embryos at corresponding assay. Embryo orientations: 50% Epiboly stage, top view with the dorsal oriented at the right; 8-somite, dorsal view with anterior oriented at the top; Prim-5 stage, lateral views with anterior oriented toward the left. Arrows show the normal location of PGCs, arrowheads demonstrate the aberrant position of PGCs induced by overexpressed DYRK1A. The numbers indicated in each picture are the number (left) of affected embryos with phenotype similar to what is shown in the picture and the total number (right) of observed embryos. The same number labeling was used thereafter.