Fig. 3
Immunohistochemical examination of ICC in WT and Tg(fabp10:nras 61K) transgenic zebrafish at 12 mpf. Immunohistochemical images of PCNA (A), CK19 (C), phosphorylated ERK (pERK) (E), phosphorylated MEK1/2 (pMEK1/2) (G) in WT and Tg(fabp10:nras 61K) transgenic zebrafish at 12 mpf. Tumor surrounding tissue of Tg(fabp10:nras 61K) transgenic zebrafish liver showing intense PCNA (B), CK19 (D), pERK (F), pMEK1/2 (H) immunostaining in Tg(fabp10:nras 61K) transgenic zebrafish at 12 mpf. Immunostaining signals in the bile duct of Tg(fabp10:nras 61K) transgenic zebrafish liver are indicated by arrowheads. Scale bars: 50 µm.