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Fig. s3 Negative staining with anti-RL-TGR antibody in cichlid embryos (Maylandia zebra) that do not have rltgr gene. (a) Confocal image showing 3 dpf wild type cichlid embryos immunostained with anti-RL-TGR antibody (green). (b) Confocal image showing 7 dpf wild type cichlid embryos immunostained with anti-RL-TGR antibody (green). There was no green fluorescence detected in both stages in cichlid because cichlid genome do not contain rltgr gene and therefore do not have RL-TGR protein. This negative control further validates the specificity of anti-RL-TGR antibody in reacting with RL-TGR protein.
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