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Fig. 7 Nuclear movement is regulated by non-Smad JNK signaling independent of Smad signaling. (A-B′) Transverse sections of ndr1 mRNA>YSL embryos treated with DMSO as control (A,A′) and SP600125 (B,B′); the cell membrane, nucleus and sox32 mRNA were visualized by β-cat staining, DAPI staining and FISH, respectively at 4.7 hpf. Arrows indicate nuclei in MMCs. (C,D) Mean DNB and DCN values in MMCs of ndr1 mRNA>YSL embryos treated with SP600125 or DMSO at 4.7 hpf. n, number of nuclei examined. ***P<0.001, **P<0.05. (E,F) WISH of sox32 expression in nlacZ or dn smad2 mRNA-overexpressing embryos at 5.3 hpf. The number of embryos is shown bottom right. Animal pole views. (G) sox32 expression in nlacZ or dn smad2 mRNA-overexpressing embryos at 5.3 hpf measured by qPCR. Expression level of nlacZ-overexpressing embryos is set as 1.0. **P<0.05. Error bars represent s.d. of three independent experiments. (H,I) Transverse sections of nlacZ- and dn smad2-overexpressing embryos; the cell membrane and nuclei were visualized by β-cat and DAPI staining, respectively at 4.7 hpf. (J,K) DNB and DNC in LMCs were measured in nlacZ- and dn smad2-overexpressing embryos at 4.7 hpf. n, number of nuclei examined. Scale bars: 10 µm in A-B′,H,I; 200 µm in E,F.