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Fig. 4 Sildenafil and vardenafil reduce adult rod outer segment shedding. (A) Schematic timeline for examining rod outer segment renewal in adult, 6 days post heat shock (dpHS) Tg(Xla.rho:EGFP); Tg(hsp70:HA-mCherryTM); albb4/b4 fish after bathing in phosphodiesterase inhibitor. (B) Representative images of photoreceptor layer from inhibitor-bathed adults immunolabeled with anti-GFP (green), anti-HA (red), and anti-Rhodopsin (blue) antibodies. Images are projections of a subset of z-sections totaling 5.97 μm. Scale bar: 10 μm. Quantification of (C) distance to shed (DS) and (D) growth distance (DG) in inhibitor-bathed adults. Lower and upper hinges of box correspond to first and third quartiles; middle corresponds to median; whiskers extend 1.5 * interquartile range above and below the hinges; dots represent outliers. Graphs represent (C) n = 3 fish/treatment (123 DMSO, 102 sildenafil, 133 vardenafil outer segments) and (D) n = 3 fish/treatment (176 DMSO, 192 sildenafil, 171 vardenafil outer segments). *95% CI of difference compared to control does not span zero.