ZFIN Image: Campbell et al., 2017, Fig. 1

Image

Figure Caption

Fig. 1 Transgenic fluorescent marker is a tool for measuring rod outer segment renewal. (A) Schematic representation of a Tg(Xla.rho:EGFP); Tg(hsp70:HA-mCherryTM) rod photoreceptor with HA epitope– and transmembrane domain–tagged mCherry (red stripe) that has inserted into nascent rod discs and displaced toward the outer segment distal tip during the several days following a heat shock pulse. Growth distance (DG) is measured from the base of the outer segment to the mCherry stripe; shedding distance (DS) is measured from the mCherry stripe to the distal tip of the outer segment. (B, C) Representative z-projection images with DS measurements from (B) 8 dpf, 3 days post heat shock (dpHS), and (C) adult, 6 dpHS. Measurements are made in the three-dimensional z-stack. Photoreceptors express GFP (green) and are labeled with anti-Rhodopsin antibody (blue).

Acknowledgments

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