|
Fig. 3
OTG inhibits Wnt/β-catenin signaling pathway during early development. (A–C) Views from animal pole, dorsal to the right. Expression pattern of dha in control (A, 10/10, 100%), wnt8 mRNA-injected (B, 14/15, 93%), or wnt8 and OTG mRNA-injected embryo (C, 12/13, 92%) at sphere stage (4 hpf). Arrow indicates Wnt8-induced ectopic expression of dha. (D–F) Morphology of control (D, 10/10, 100%), wnt8 mRNA-injected (E, 18/20, 90%), or wnt8 and OTG mRNA-injected embryo (F, 17/20, 85%) at 30 hpf. (G–I) Morphology of control (G, 12/12, 100%), LiCl treated (H, 13/15, 86%), or LiCl treated and OTG mRNA-injected embryo (I, 14/15, 93%) at 24 hpf. (J–M) Views from animal pole, dorsal to the right. Expression pattern of tbx6 (J, 10/10, 100%; K, 18/20, 90%) or dkk1 (L, 10/10, 100%; M, 17/20, 85%) in control (J,L) or OTG mRNA-injected (K,M) embryo at 6 hpf.