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Fig. s8 (a) Immunohistochemistry (IHC) for AT270 (red) and GFP (green) on coronal sections of telencephalon of a 6-month old nontransgenic animal. (a’, a’’) Individual fluorescent channels for GFP (a’) and AT270 (a’’). Insets show the enlarged view of the frame in a with individual channels for DAPI, GFP and AT270, and merged image. (b) IHC for AT270 and GFP on coronal sections of telencephalon of a 6-month old dTg animal. (b’, b’’) Individual fluorescent channels for GFP (b’) and AT270 (b’’). Insets show the enlarged view of the frame in b with individual channels for DAPI, GFP and AT270, and merged image. (c) Western blot for AT270 from brains of non-Tg, sTg and dTg animals. Scale bars equal 50 μm. n = 6 fish for every staining.