Fig. 2

Fig. 2

(a) Immunohistochemistry (IHC) for L-Plastin (red) and TAU^P301L (green) on coronal sections of telencephalon of a 6 month-old sTg animal. Single channel images of the whole section for TAU^P301L (a’) and L-Plastin (a”). (b) The enlarged view of the inset in a. (c) IHC for L-Plastin (red) and TAU^P301L (green) on coronal sections of telencephalon of a 6-month old dTg animal. Single channel images of the whole section for TAU^P301L (c’) and L-Plastin (c”). (d) The enlarged view of the inset in c. (e) Quantification of round and ramified L-Plastin-positive cells in the telencephalon in sTg and dTg animals. (f) Immunohistochemistry (IHC) for S100β (red), PCNA (green) and TAU^P301L (white) on coronal sections of telencephalon of a 6-month old sTg animal. Single channel images of the whole section for S100β (F’), PCNA (f”), and TAU^P301L (f”’). (g) IHC for S100β (red), PCNA (green) and TAU^P301L (white) on coronal sections of telencephalon of a 6-month old dTg animal. Single channel images of the whole section for S100β (g’), PCNA (g”), and TAU^P301L (g”’). (h) The enlarged view of the inset in f. (i) The enlarged view of the inset in g. (j) Quantification of the total number of proliferating glial cells in the telencephalon of sTg and dTg animals. Values represent mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.005. Scale bars equal 50 μm (a–g”’) and 20 μm (h,i). n = 7 fish and > 30 histological sections for every staining. All animals are 6 months old.