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Fig. 3
Hyperglycemia induced muscular disarray, myofibril loss and apoptosis activation. (A,A?) H&E staining of ventricle sections showed muscular disarray and myofibril loss in treated fish (A?) compared with the wild-type group (A) after 32 weeks of treatment (n=20 field, repeated five times); scale bars: 100??m. (A?) Quantification analysis of ventricular myocardial density in H&E-stained hearts between the two groups in weeks 8 and 32. (B,B?) TUNEL (green)-stained sectioned ventricles co-stained with Mef2 (red) of wild-type (B) and treated (B?) fish in week 32 (n=20 field, repeated five times); scale bars: 10??m. Arrows: TUNEL+/Mef2+; arrowheads: TUNEL+/Mef2?. (B?) Measurement of the ratio of apoptotic nuclei [yellow (green plus red)] to total myocardial nuclei (red) between the two groups in weeks 8 and 32. (C,C?) Longitudinal TEM image verified muscular disarray and myofibril loss detected in the hearts of the treated fish (C?) compared with that in the wild-type group (C) (n=20 field, repeated five times); scale bars: 2??m. (C?) Transverse TEM image showed myofibril loss (arrows) in the hearts of treated fish. Inset is a higher magnification image. Scale bar: 2??m. (A?,B?) Values are meansąs.d. **P<0.01 compared with the wild-type group. n, number of fish examined.