ZFIN Image: Zhang et al., 2017, Fig. 3

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Fig. 3

Distinct early and late roles of Notch signaling in regulating duct and endocrine lineages. a, b Pancreas of 60 hpf jag1b ^−/− ;jag2b ^−/− and wt sibling embryos. Neogenic endocrine cells expressing Islet1 (red) are normally found outside the principal islet at the base of the wild type pancreas (arrows, a). These neogenic endocrine cells are not present in the 60hpf jag1b ^−/− ;jag2b ^−/− mutant pancreas (b, representative sample, n = 18, pooled from three different jag1b ^−/+ ;jag2b ^−/+ in-cross clutches), which is entirely ptf1a:GFP⁺ (green) with the exception of the principal islet. c, d Pancreas of 5 dpf-treated embryos. Early inhibition of Notch signaling with gamma secretase inhibitor LY575411 treatment (d, n = 9 and c, n = 5. Samples from 2 different experiments) at 30–72 hpf leads to loss of Nkx6.1⁺ pancreatic duct (red) without excess neuroD:GFP⁺ (green) endocrine cells. e, f In contrast, late LY575411 treatment (f, n = 9, and n = 6 for control, e Samples pooled from 2 different experiments) also leads to loss of nkx6.1⁺ pancreatic duct (red) that is coupled with excess neuroD:GFP⁺ (green) endocrine cells (arrowheads, f). Scale bar, 50 μM

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Acknowledgments

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