Fig. 2

Fig. 2

Dynamics of Ca²⁺ signaling in Tg[βactin2:GCaMP6s]^{stl351/stl351} embryos at cleavage and blastula stages. (A-L) Still images of GCaMP6s signals during cleavage furrow progression from 2-cell stage to 16-cell stage. (M) Quantification of Ca²⁺ transient duration before and after MBT. Error bars represent standard deviation; N=4 embryos. ns, not significant. (N) Comparison of Ca²⁺ transient numbers before and after MBT; N=6 embryos. (O-P) Still images and traces of Ca²⁺ transients in a time-lapse series at blastula stages. (Q) Time-lapse overlay of GCaMP6s signal from 3.7 hpf to 4 hpf from a single z-section in lateral view.