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Fig. 1
FIGURE 1. Main steps to obtain a highly pure adult zebrafish spinal oligodendrocyte progenitor cell (OPC) population: (1) Adult zebrafish spinal cord tissue is dissected. (2) The tissue is first dissociated by enzymatic digestion and then incubated with a nuclear dye. (3) The cell suspension is FACsorted based on cytoplasmic and nuclear fluorescence. (4) The purified OPCs can then be used directly for transcriptome analysis, cell culture as monoculture to study dormant OPCs or in motor neuron/OPC co-cultures to study oligodendrocyte differentiation.
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Full text @ Front. Cell. Neurosci.