ZFIN Image: Seritrakul et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Fig 3. tet2^-/-;tet3^-/- retinal cells do not undergo terminal differentiation.

(A,F) HuC/D labels RGCs and amacrine cells (ACs), which are reduced in number and located only in the central region of the INL in tet2^-/-;tet3^-/- retinae (arrow). (B,G) tet2^-/-;tet3^-/- mutant retinae almost entirely lack zpr-1⁺ red/green cones (arrow); (C,D,H,I) possess few zpr-3⁺ rods (arrow), and of those that are zpr-3⁺, outer segments are severely attenuated or almost absent (arrow). (E,J) tet2^-/-;tet3^-/- retinae also possess few zrf-1⁺ Müller glia (arrows in wild-type). In all cases, marker⁺ cells are located in the central/ventral part of the retina. (K,P) Zn8 detects neurolin, a protein enriched on RGCs and the optic nerve (arrowhead in K). (L,Q) Zn8 staining reveals the optic nerve in the choroid fissure and optic chiasm (arrow) of wild-type embryos but not in tet2^-/-;tet3^-/- mutants. (M-N) isl2b:GFP transgenics express GFP in RGCs and PRs, clearly labeling the optic nerve in whole-mount and section views (arrowhead). (R,S) The tet2^-/-;tet3^-/- optic nerve is very thin, often unilaterally formed, but, when present, correctly routed to the brain. (O,T) The isl2b:GFP signal overlaps zpr-3 (rod) marker in the cell body and outer segments in siblings (arrows). In tet2^-/-;tet3^-/-, few isl2b:GFP⁺ cells are zpr-3⁺ (arrows), further suggesting that specified cells are not terminally differentiated. Few outer segments have also formed in tet2^-/-;tet3^-/- mutants. DNA (blue), antibody stain (red). All images are 3dpf. n>5 for each marker. Dorsal is up and anterior to the left. Scale bar = 80μm in A-K, P.

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Acknowledgments

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