ZFIN Image: Botthof et al., 2017, Fig. S2

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Figure Caption

Fig. S2

Loss of rad51 causes DNA damage sensitivity. (A) Chromosome spreads of 24 hpf wild type and mutant embryos treated with 5 μg/ml mitomycin C for 20 hours. White arrows indicate premature chromatid separation events (i). Quantification of the damage (ii). Mann- Whitney test, P=.001, n_+/+=16, n_-/-=19. (B) Chromosome spreads of 24 hpf wild type and mutant embryos treated with 1 nM CPT for 20 hours. White arrows indicate breaks and radial structures in response to topoisomerase inhibition (i). Quantification of the damage (ii). Mann-Whitney test, P<.0001, n_+/+=12, n_-/-=17. (C) Chromosome spreads of 24 hpf wild type and mutant embryos treated with 10μ μM DiQ for 20 hours (i). Quantification of the damage (ii). Mann-Whitney test, P=.13, n_+/+=14, n_-/-=29. Bars represent mean +/- SEM in all graphs.

Acknowledgments

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