ZFIN Image: Botthof et al., 2017, Fig. 4

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Figure Caption

Fig. 4

The rad51^sa23805 HSPC defect starts during embryonic development. (A) ISH using a cmyb-specific probe at 2 dpf; the arrow shows HSPCs. Representative images of the three different staining categories are shown (i) and a quantification of the different genotypes (ii) n = 119 from two clutches. (B) ISH using a cmyb-specific probe at 4 dpf; the arrow shows HSPCs. Representative images of the three different staining categories are shown (i) and a quantification of the different genotypes (ii), n = 120 from two clutches. (C) Quantification of BrdU⁺ cells in the tail at 2 dpf. Two-sided Student’s t test, P = 0.042, n^+/+ = 3, n^−/− = 3. (D) Quantification of BrdU⁺ cells in the CHT at 4 dpf. Two-sided Student’s t test, n^+/+ = 4, n^−/− = 4. Bars represent mean ± SEM in C and D. n.s., not significant. (E) Representative images of TUNEL-stained 2 dpf embryos from a rad51^+/− in-cross. Dotted lines indicate the area of the CHT that was scored. Arrows indicate TUNEL⁺ cells. (F) Quantification of three clutches of TUNEL-stained 2 dpf rad51^+/− in-crosses. Each clutch was scored blindly and consisted of 10^+/+ and 10^−/− embryos each. Shown is the mean of all clutches ± SEM. (Magnification, 100× in all images.)

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Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA