ZFIN Image: Botthof et al., 2017, Fig. 1

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Figure Caption

Fig. 1

The rad51^sa23805 allele leads to loss of Rad51 protein and causes DNA damage sensitivity. (A) Western blot showing the expression of Rad51 in testes extracts of WT and mutant zebrafish. β-Actin was used as a loading control. (B) Representative Rad51 immunostained embryos derived from a rad51^+/− in-cross. Secondary only (i), Abcam primary (ii), AnaSpec primary (iii). (Magnification, 65×.) (C) Chromosome spreads of 24-hpf WT and mutant embryos treated with 1 µg/mL DEB for 20 h taken using a 100× oil-immersion objective. White arrows indicate characteristic damage (chromosome breaks and radial structures) in response to cross-linking agents (i). Quantification of the damage (ii). Mann–Whitney test, P = 0.0004, n^+/+ = 25, n^−/− = 26. (D) Comparison of the response of 48-hpf WT and mutant embryos to irradiation (i). (Magnification, 15×.) The black arrow indicates the small head and eye phenotype, which is quantified in ii. Two-tailed Fisher’s exact test pooling WT and heterozygotes as control group, P < 0.001, n = 67. (E) Immunostaining for pH2AX in WT and mutant embryos with pictures of representative embryos taken with a 63× water-immersion objective (i) and quantification of foci (ii). White arrows indicate example foci. Two-tailed Student’s t test, P < 0.0001, n^+/+ = 8, n^−/− = 11.

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Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA