ZFIN Image: Choi et al., 2018, Fig. 6

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Figure Caption

Fig. 6

Increase of mRNA expression of stress-related crhb in sam2^cnu1 KO fish and spontaneous inhibitory postsynaptic currents onto CRH neurons by SAM2. (A–D) Increase of crhb mRNA expression in sam2^cnu1 KO fish (sam2^+/+, n = 6; sam2^−/−, n = 6). Ventral views of the whole brain of control sam2^+/+ (A and C) and sam2^−/− KO fish (B and D). (A′ and B′) Higher magnifications of the boxed regions in A and B are the PPa in zebrafish, homologous to the mouse PVN. (Scale bars, 200 µm.) (E) Representative photomicrograph of the Cre-dependent TdTomato in the PVN CRH neurons. (Magnification: E, 10×.) (F) Representative voltage-clamp traces of sIPSCs in response to SAM2 application in PVN CRH neurons. (G) SAM2 application significantly increased sIPSC frequency. (H) SAM2 did not affect sIPSC amplitude. (I) Representative voltage-clamp trace of eIPSC onto CRH neurons in response to SAM2 application. (J) SAM2 did not affect the amplitude of eIPSCs. (K) SAM2 did not change the paired-pulse ratio. *P < 0.05; **P < 0.01.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA