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Fig. 7 S1
Collagen disruption impaired regeneration following amputation of the caudal fin.
(A) Inhibition of the collagen crosslinking enzyme lysyl oxidase with BAPN resulted in reduced (B) regenerate area and (C) regenerate length at 2 dpw. (D) SHG image analysis of BAPN treated larvae at 24 hpw revealed a change in both (E) wound contour length and (F) fiber mean angle. (G) The change in caudal fin length was less in burn treatment than tail transection as between 24 and 48 hr after injury. Data in B and C represent a single experiment performed in triplicate. Data in E, F, and G are pooled from three replicate experiments. Scale bars represent 100 µm. ****p<0.0001.