ZFIN Image: Singh et al., 2017, Fig. S20

Image

Figure Caption

Fig. S20

Glucose-stimulated calcium fluxes in beta-cells at 45 dpf. (A-B) Glucose-responsiveness of larval beta-cells at 45 dpf. (A) Islets from Tg(ins:GCaMP6s); Tg(ins:mKO2-nls) animals were mounted ex-vivo and live-imaged while providing increasing concentration of glucose from 5 mM (basal) to 10 and 20 mM, followed by addition of 30 mM KCl. The islets were divided into two halves along the anterior-posterior (A/P) axis, as indicated by the white dotted line (anterior is to the top). Representative cells within each half are marked with arrowheads (anterior – blue; posterior – red). (B) The fluorescence-trace for both cells is shown below in their respective colors. Both cells responded to the stimulation with glucose, as indicated by the oscillating GCaMP6s fluorescence-intensity. Scale bars, 20 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.