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Fig. S2
The hindbrain angiogenic vasculature of wild-type and zygotic ikk2-/- embryos (obtained from heterozygotic parents) at 48 hpf. (A-F), ImageJ processed binary image of the confocal projection view of the hindbrain CtA vessels. I'-G', corresponding output image from ImageJ Angiogenic Analyser Macro with corresponding vessel junctions, branches, master segments, isolated segments marked in different colours. Their length and numbers were analysed to calculate the angiogenic index using ImageJ Angiogenic Analyser Macro as shown in Figure 3, F-H.
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