ZFIN Image: Kesavan et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Targeted knock-in of tRFP into the otx2 locus. Expression of tRFP at 24 hpf. Images were taken from live embryos anesthetized in MS-222. (A) Left panel shows a dorsal view with the anterior of the embryo facing upwards, right panel shows a merged image of fluorescent and transmitted light channels. tRFP is expressed in the retina and midbrain, sharply abutting the MHB (dotted line). (B) Lateral view of embryos expressing tRFP. Left panel shows fluorescent channel and right panel shows a merged image of the fluorescent and transmitted light channels. Images are maximum intensity projections covering 50 μm tissue with a Z-interval of 2 μm. MB, midbrain; Hb, hindbrain. Scale bar: 100 μm.

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Expression / Labeling details

Acknowledgments

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