ZFIN Image: Lin et al., 2017, Fig. 4

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Figure Caption

Fig. 4

Overexpression of klf8 mRNA caused bilateral expression of Nodal signalling component genes. Injection of klf8 mRNA induced bilateral expression of spaw in the LPM (arrow) at the 18 s stage, in a dose-dependent manner (a-e). Embryos injected with klf8, but not LacZ, exhibited bilateral expression of lft1 in the diencephalon (arrowhead) and heart (asterisk; f-j), lft2 in the heart (asterisk; k-o), and pitx2 in the LPM (white arrow) (p-t) at the 19–22 s stage. Expression of ntl in the notochord is similar in wild type and embryos injected with LacZ or klf8 mRNA (u-w). A, absent; B, bilateral; L, left; R, right

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