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Fig. S8
Overexpression of cpn1 alters the expression of vascular markers. The expression of vascular markers was examined by in-situ hybridization (A-J) and/or quantitative real-time PCR (K). Compared to the wild type controls at 24 hpf (A, C, E, G, I), the expression of the venous/ISV markers flt4 (D, K), mrc1 (F, K) and dab2 (K) was decreased in overexpressed cpn1 embryos (cpn1mRNA); the expression of the pan-vascular markers stabilin (H), and flk (J, K) was no-to-slightly increased in embryo; the expression of the arterial marker ephrinb2 is unchanged (B, K). (K) Quantitative results by qPCR showed the expression of vascular markers ephrinb2 (1.1 ± 0.2), mrc1 (0.64 ± 0.05), dab2 (0.62 ± 0.12) and flk1 (1.4 ± 0.3),) in overexpressing cpn1 embryos. Values on the top right indicate the number of embryos exhibiting phenotype per total number of embryos analyzed. Scale bars are 200 μm. dorsal aorta (a); vein (v), and caudal vein plexus (cvp). Data are represented as means ± S.D. ** refers to p < 0.001 and * refers to p < 0.05 by an unpaired Student's t-test.