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Fig. S7
Knockdown of cpn1 can be rescued by overexpression of cpn1. In uninjected control embryos, ISV has reached the dorsal region and formed DLAV around 28– 30 hpf ((A), arrowheads). At the same stage in cpn1 MO, ISVs have stalled or slowed growth at mid-somite ((C), hollow arrowheads). Overexpression of cpn1 by cpn1mRNA (100pg) injection had no obvious defect in vasculature (B), but rescued the defect of ISV stalling ((D), solid arrowheads). (E) Quantification of percentage of completed ISV at 30 hpf shows a ~35% increase in rescued embryos compare to cpn1 morphants. Percentages of completed ISV are ~90 ± 9, 88 ± 13, 35 ± 20, and 70 ± 15 in control, cpn1 mRNA overexpression, cpn1 MO, and rescued embryos, respectively. (*** refers to p < 0.0001 by an unpaired Student's t-test. Data represent means ± S.D). Scale bars are 100 μm for (A–D).