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Fig. S5
Gross developmental process is unaffected in cpn1 morphants. Knockdown of cpn1 did not alter the expression pattern of heart, gut, somite and neural systems by examining the expression of cmlc2 (heart marker, h; A-B are lateral view and A'-B' are ventral view), gata6 (heart and gut marker, C, D), myoD (somite marker, s, E, F), and sox3 (neural tube marker, nt, G, H). Those probes have been described and documented in ZFIN. (I) Knockdown of cpn1 did not cause developmental delay by measuring heart rate. Quantification of zebrafish heart beats per minute in uninjected control and cpn1 morphants shows no difference (n=16 in control and n=19 in cpn1 MO) at 24-25hpf. The mean heart rate determined by direct visual examination of ventricle beating in control was 100.7 ± 6.2 beats per minute, 100.4 ± 10.3 beats per minute in cpn1 MO. The scale bars represent 250 μm in A-H.