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Fig. 8
SGK1 inhibition rescues a zebrafish model of long QT. (A) Still frame image of zebrafish heart with chamber labels (A?=?atrium) (V?=?ventricle). Representative tracings of chamber wall position demonstrating 2:1 AV block in mutant bkd ?/? zebrafish (upper panel) and with rescue of the phenotype (lower panel). (B) Knock-down with SGK1 morpholino or inhibition of SGK1 (500??M SGK1-DN mRNA) in the breakdance zebrafish mutants with long QT at 1?2 cell stage leads to significant rescue of 2:1 AV block at 99 hpf stage (5% spontaneous reversion at this stage); *p-value?<?0.05 by Z-test. (C) Compound 5377051 added at 48?hours post fertilization (hpf) and assessed at 72 hpf for rescue of phenotype 2 with superimposed rescue (solid line) and toxicity (dashed lines) curves shown on left (C) and representative bar graph on right (D). *p?<?0.05 by Z-test.