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Fig. 8
Expression of sept3, sept5a and sept5b in substructures of the adult zebrafish brain. The expression pattern of sept3 (A1–A5), sept5a (B1–B5), and sept5b (C1–C5) is shown in sagittal sections of adult brains. The regions of the brain are indicated in the scheme (1–5). In the telencephalon (1) sept3, sept5a and sept5b are expressed in dorsal telencephalic areas (Dm, Dc and Dp) and ventral telencephalic areas (Vd and Vv). Septins are expressed in a stream in the Vd region (outlined by dotted line). Additionally, sept3, sept5a and sept5b is expressed in the Ob. The sept3 and sept5b positive layer in the TeO is labeld with black arrowheads (A2,C2). In the cerebellum (3,5) sept3, sept5a and sept5b is expressed in the LCa, and in the region of the purkinje cell layer (PCL). Sept3 is additionally expressed in the CCe. Furthermore, hypothalamic sagittal section are shown (4). Abbreviations can be found in Table 1. To identify dopaminergic cells, ISH stained brains (magenta) against sept3 (D), sept5a (E), and sept5b (F) were counterstained with an antibody against tyrosine hydroxylase (TH, green in D1,E1,F1) and analyzed with a confocal microscope and merged (D2,E2,F2). Pictures in (D–F) are maximum projections of confocal slices through adult sagittal brain sections. Scale bars 200 μm.