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Fig. 5
Transverse sections through 4 dpf zebrafish larvae stained with ISH probes against sept3, sept5a and sept5b. 2 dpf zebrafish larvae were stained for sept3 (A,B,G,H,M,N,S,T), sept5a (C,D,I,J,O,P,U,V) and sept5b (E,F,K,L,Q,R,W,X), embedded in epon, and cut in 8 μm thick section. For better orientation, the ISH sections were counterstained with Hoechst (A,G,M,S,C,I,O,U,E,K,Q,W). Septin expression is indicated by the blue color (B,H,N,T,D,J,P,V,F,L,R,X). The tectum (TeO), preoptic region (Po), thalamus (Th), hypothalamus (Ht), cerebellum (Ce), the medulla oblongata (Mo) and the otic capsules (Oc), are marked. Additionally the migrated posterior tubercular area (M2) and the posterior tuberculum (Pt) are annotated. In the eye, the retinal ganglion cell layer (Rgl), the inner and outer nuclear layer (Inl and Onl), as well as the inner and outer plexiform layer (Ipl and Opl) are shown. The ventricles are indicated with white arrowheads. Schemes for better orientation with indicated section planes are modified from Mueller and Wullimann (2015) 5 dpf schemes, as these are structurally very close to the here shown 4 dpf sections.