ZFIN Image: Mei et al., 2014, Fig. 5

Image

Figure Caption

Fig. 5

pk2 knockdown suppresses the bbs7-related retrograde melanosome transport defect. (A) Diagram of melanosome transport in melanocytes by the intracellular transport machinery. Dark-adapted are dispersed by anterograde transport while in response to light, they move to the perinuclear region by retrograde transport. (B)–(B′) 5 dpf Zebrafish larva before and after epinephrine treatment. Epinephrine stimulates retrograde transport while caffeine stimulates anterograde transport. (C) Graph of retrograde transport time in different groups. bbs7 knockdown leads to a delay in the retrograde transport while pk2 knockdown alone does not. However, the double knockdown larvae show a suppression of the retrograde transport delay. Uninj, n=50; bbs7 MO, n=52; pk2 MO, n=50; bbs7 MO+pk2 MO, n=51. Error bars are presented as standard error. p<0.01; ANOVA with Tukey.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 392(2), Mei, X., Westfall, T.A., Zhang, Q., Sheffield, V.C., Bassuk, A.G., Slusarski, D.C., Functional characterization of Prickle2 and BBS7 identify overlapping phenotypes yet distinct mechanisms, 245-55, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.