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Fig. 4 S5
Morpholino inhibition of ptger2a fails to affect proximal segment identity.
(A) Embryos were microinjected with ptger2a MO1 or MO2 and grown until 24 hpf. WISH was used to visualize the PCT (slc20a1a) and PST (trpm7) (purple) and the somites (red) at the 24 hpf stage. Black bars indicate segment gene expression domain. Red scale bar, 70 µm. (B) Segment areas for the PCT, PST, DE, and DL were quantified in triplicate using parallel somites. Greater than (>) and less than (<) were used to categorize embryos, where each (>) or (<) represents the difference of a somite in segment area. At least 20 embryos were used per control and experimental group. Data are represented as ± SD significant by t test comparing each drug treatment to the corresponding DMSO control group, *p<0.05, **p<0.005, where n.s. indicates not significant.