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Fig. 4 S3
Inhibiting prostaglandin synthesis with splice morpholinos promotes distal early identity.
(A) WT embryos were microinjected with either ptgs1 MO splice or ptgs2a MO splice and grown until 24 hpf. WISH was used to assay for the PCT (slc20a1a), PST (trpm7), DE (slc12a1), and DL (slc12a3) (purple) and the somites (smyhc1) (red) at the 24 hpf stage. Black bars indicate segment gene expression domain. Red scale bar, 70 µm. (B) A somite summary map depicting the changes induced by ptgs1 MO splice and ptgs2a MO splice microinjection.
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