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Fig. 3
Prostaglandin production is required for normal distal cell fate specification.
(A) Embryos were treated with 0.3% DMSO or the nonselective Cox inhibitor indomethacin at 30 μM from 4 hpf to 24 hpf. WISH was used to stain for the PCT (slc20a1a), PST (trpm7), DE (slc12a1), and DL (slc12a3) (purple) and the somites (smyhc1) (red) at the 24 hpf stage. Black bars indicate segment gene expression domain. Red scale bar, 70 µm. (B,C) The DE and DL segments were measured in microns (n = 5 for each control and experimental group). (D) A summary depicting the nephron segments after indomethacin treatment. Data are represented as ± SD, significant by t test comparing each drug treatment to the DMSO control group, **p<0.005.