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Fig. S7
Distinct subpopulations of ventral cilia in each brain subdivision increase in length during neurulation.
Tg(?-actin:mGFP) embryos stained with antibodies against acetylated ?-tubulin (green), gamma-tubulin (blue) and GFP (red), except in A, where nuclei are stained red. (A-C) Cilia in the ventral FB do not change in length over time. (D) Average length of ventral FB cilia at 12S (1.36?m), 18S (1.87?m) and 24S (1.69?m). (E-G) Ventral MB cilia are longer than primary cilia on average and increase in length over time. (H) Average length at 12S (2.76?m), 18S (4?m) and 24S (5.77?m). (I-K) Ventral HB cilia are longer than MB cilia at 12S and increase in length over time. (L) Average length at 12S (3.72?m), 18S (5.02?m) and 24S (5.01?m). A-C, E-G, I-K are stacked confocal images with anterior to the left. Error bars represent standard error of the mean.