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Fig. S4
Morpholino designed against the translation start site of Rsph9 blocks translation from exogenous Rsph9 RNA.
A-D: Wild-type embryos injected with different combinations of rsph9 MO and rsph9- tRFP mRNA. A: Injection of rsph9-tRFP mRNA alone results in tRFP fluorescence at varying levels between embryos. B: Co-injection of rsph9MO with tRFP mRNA does not prevent tRFP fluorescence. C,D: Co-injection of rsph9MO with rsph9-tRFP mRNA, which contains the binding site of the rsph9MO, prevents expression of rsph9-tRFP, as indicated by absence of RFP fluorescence.
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