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Fig. S2
Control experiments for morpholino specificity.
The specificities of the MOs against ccndx were confirmed using pCMV-GFP reporter plasmids containing the MO target sequence. The pCMV-GFP reporter plasmids bearing the MO target sequence were injected into zebrafish embryos together with the control MO or ccndx ATG MO (A, panels a and a'). All images were taken from zebrafish embryos at 48 hpf. (B) Zebrafish embryos were injected with splice-blocking MOs against ccndx. The efficiency and specificity of ccndx splicing MO were confirmed by RT-PCR with two primer sets (panel a); the RT-PCR results are illustrated in panel b.