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Fig. S7 Phenotypes of Dtx1 morphants were not caused by nonspecific p53 activation. Embryos coinjected with dtx1 MO and tp53 MO were compared with Dtx1 morphants, and we observed no detectable deviation from all markers tested. Compared with the controls, the injection of tp53 MO alone did not show any significant alterations, as shown by in situ hybridization (a), immunohistochemistry (b), qPCR (c), and cell count (D).*, P < 0.05; **, P < 0.01; n.s., nonsignificant.
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