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Fig. 1
Phenotype of the ia2 mutant at 24 hpf.
The homozygous mutant (C) displays a strongly delayed development and compromised phenotype, while the heterozygote (B) appears indistinguishable from the wild-type (A). The green fluorescence relative to the -8.5nkx2.2a:GFP transgene is present in heterozygous and homozygous ia2 mutant fish, being the GFP gene co-inherited with the ia2 mutant allele. This allows the early and immediate screening of the three genotypes.
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