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Fig. 2
FGF signalling regulates expression of osx.
(A-H) Larvae were treated at 48hpf, fixed at 51hpf and expression of several bone markers was analysed by in situ hybridisation. Arrowheads point to the opercle in the high magnification images to the right of each panel. Expression of runx2a (A-D) and runx2b (E-H) is unchanged after inhibition of FGF signalling using hs:dnfgfr1 larvae as well as over activation in hs:fgf3 transgenic fish. Expression of runx2b is slightly reduced after SU5402 treatment (F). (I-L) Expression of osx is decreased after inhibition of FGF signalling and increased after over activation of FGF signalling as shown by in situ hybridization. Abbreviations: cl = cleithrum, op = opercle. Scale bar = 200μM. (M) Quantification for presence or absence of osx staining in the opercle from I-L. Expression was normalised to the control. (N) qPCR performed in parallel to I-L confirm effects of FGF signalling on osx expression. Treatments were normalised to gapdh. Treatment with SU5402 showed a significant reduction (p<0.05 Student's t-test).