ZFIN Image: Sun et al., 2017, Fig. 9

Image

Figure Caption

Fig. 9

Epiboly and actomyosin formation are defective in MZalkbh4;MZatrn double mutant embryos. (A) Epiboly initiation and progression delay in MZalkbh4;MZatrn double mutants. (B) The quantitative data of epiboly progression at 8 hpf are shown. (C) Co-IP assay shows that endogenous Actin-NMII interaction is decreased in alkbh4 and atrn double morphant embryos. Embryos at 75% epiboly stage were harvested for lysis. (D) Confocal images of phalloidin stained embryos at 50% epiboly, and phalloidin and anti-NMII double stained embryos at 75% epiboly. (E) Quantitative measurements of actomyosin band widths in (D) at 75% epiboly stage. (F) Quantitative measurements of the marginal EVL cell length/width ratios in (D) at both 50% and 75% epiboly stages. Ne, the number of observed embryos; Nc, the number of observed cells. *, p<0.01. Scale bars: 100 μm in (A); 50 μm in (D).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Biol. Sci.