ZFIN Image: Sun et al., 2017, Fig. 2

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Fig. 2

Morpholino knockdown of alkbh4 leads to epiboly initiation and progression delays. (A) Effectiveness of alkbh4 morpholinos. 50 pg alkbh4-5'UTR-GFP plasmid DNA was injected alone or with 10 ng std-MO, alkbh4-MO1 or alkbh4-MO2 at 1-cell stage. The injected embryos were observed for GFP expression at late gastrulation stages. (B) Morpholino knockdown of alkbh4 leads to epiboly delay in a dose dependent manner. Embryos were observed and photographed at indicated stages. Magnified observations of the embryonic marginal cell layers are shown at the last panel. (C) Epiboly initiation in (B) was measured by the percentage of embryos with yolk cell doming at 5 hpf. (D) Epiboly progression in (B) was measured by the average epiboly percentage of the embryos at 8 hpf. Ne, the number of observed embryos. *, p<0.01. Scale bars: 500 μm in (A); 100 μm in (B).

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Acknowledgments

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