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Fig. S6
Wild-type and mutant gonads have low levels of membrane-associated Cdh1/E-cadherin at 10 dpf.
(A-D') Single plane confocal micrographs of whole-mount larval gonads immunostained for Cdh1/E-Cadherin. In most 10 dpf wild-type (WT; A, A'; 10/15) and fgf24 mutant (D, D'; 10/10) animals, Cdh1 (red) does not localize to cell membranes of gonadal cells. In some cases, wild-type animals have low expression of Cdh1 at the membranes of SGCs (B, B'; 3/15) or germ cells (C, C'; 2/15). A-D' are sagittal optical sections with anterior to the left. Germ cells are labeled with Vasa (green), nuclei are labeled with DAPI (blue). (A', B', C', D) Cdh1 channel only, in grey. Arrow = membrane localization of Cdh1 in gonadal cells; Asterisk = membrane localization of Cdh1 in a nearby, non-gonadal tissue. Scale bars = 20 μm.