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Fig. S5
Time course of the expression of genes shown to be downregulated by transient gbx2 induction. The expression of the two brain regionalization genes, shown by the microarray data to be downregulated, were reexamined by WISH at 0, 1, and 2 hph in sibling and hsp-gbx2+ embryos. The data show that her5 is immediately downregulated, whereas hesx1 responded much later, suggesting indirect regulation by gbx2. None, no abnormal expression was observed. Scale bars, 200 µm.
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Reprinted from Developmental Biology, 430(1), Nakayama, Y., Inomata, C., Yuikawa, T., Tsuda, S., Yamasu, K., Comprehensive analysis of target genes in zebrafish embryos reveals gbx2 involvement in neurogenesis, 237-248, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.