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Fig. S2
Whole-mount in situ hybridization (WISH) analysis of genes whose expression was shown by the microarray data to be altered by transient gbx2 induction. The expression of the genes shown by the microarray analysis (MA) to be significantly upregulated (Upregulated-MA) or downregulated (Downregulated-MA) was re-examined by WISH in embryos that were exposed to two modes of heat shock (A, 35 °C for 15 min and fixed 1 h post-heat shock (hph); B, 37 °C for 30 min and fixed 2 hph). For the genes shown here, no differences were observed, in contrast to the genes shown in Fig. 3.
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Reprinted from Developmental Biology, 430(1), Nakayama, Y., Inomata, C., Yuikawa, T., Tsuda, S., Yamasu, K., Comprehensive analysis of target genes in zebrafish embryos reveals gbx2 involvement in neurogenesis, 237-248, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.