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Fig. S6
Macrophage depletion with irf8-MO does not rescue immune phenotype. (A) Exon map of irf8 transcript depicting the location of the splice-blocking morpholino (MO), binding site (black bar) and the primers used to validate its efficacy (blue boxes). (B) Loss of full-length irf8 transcript in uhrf1 mutants after irf8-MO knock down. (C) Embryos with or without 0.6 mM irf8-MO injection were assayed for presence of macrophages using Neutral Red (D) RT-qPCR for expression of interferon gene panel in uhrf1 and (E) WT siblings at 80 hpf. A line connecting two dots represents one paired sample with siblings from a single clutch. Significance determined by paired t-test.