ZFIN Image: Xue et al., 2017, Fig. S2

Image

Figure Caption

Fig. S2

klf6a morphants display the CVP and HSPC defects. Related to Figure 2.

(A) The relative expression of klf6a in different cell populations. The kdrl:mCherry⁺ ECs, gata1:dsRed⁺ erythroid cells, and coro1a:GFP⁺ myeloid/lymphoid cells were sorted from transgenic embryos at 42 hpf. (B) Confocal imaging of Tg (kdrl:mCherry/fli1:nGFP) or Tg (kdrl:GFP) in the CHT of control, klf6a morphants and klf6a^-/- embryos 48 hpf and the statistical data of the CHT EC number at 48 hpf (mean ± SD, t-test, **P<0.01, ns>0.05). Scale bar, 50 μm. (C) Expression of ikaros, cmyb, irf4a, lyz, and be1-globin at 3 dpf or 4 dpf in control and klf6a morphants. (D) Confocal imaging of the CHT region in control, klf6a morphants and klf6a MO plus klf6a mRNA injected Tg (gata1:dsRed/mpo:GFP) embryos. klf6a morpholino (MO) or klf6a MO plus klf6a mRNA was injected into transgenic embryos. Scale bar, 100 μm. (E) qPCR result of gata1, mpo, and cmyb in control, klf6a morphants and klf6a MO plus klf6a mRNA injected embryos at 3.5 dpf (mean ± SD, t-test, *P<0.05, **P<0.01, ***P<0.001, n = 3). ns, not significant.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 42(4), Xue, Y., Lv, J., Zhang, C., Wang, L., Ma, D., Liu, F., The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b, 349-362.e4, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell