Fig. 6

Fig. 6

Ccl25b Acts Downstream of Klf6a to Facilitate HSPC Settlement and Expansion

(A) Time-lapse imaging of kdrl:mCherry/runx1:en-GFP in control and ccl25b morphants. The white arrowheads mark HSPCs and the white circles indicate HSPC pockets. Scale bar, 50 μm.

(B) The statistical data of runx1⁺ cells in D/M/V of CVP in control and ccl25b morphants at 50 hpf (mean ± SD, t test; ^∗p < 0.05, ns [not significant] > 0.05, n = 8).

(C) Confocal imaging of kdrl:mCherry/runx1:en-GFP CHT at 3 dpf in control embryos and ccl25b morphants. Yellow arrowheads mark GFP⁺ cells. The white dashed lines mark the outlines of caudal artery and vein. Scale bar, 50 μm.

(D) The quantification of runx1:en-GFP⁺ cells shown in (C) (mean ± SD, t test; ^∗∗p < 0.01, n = 8).

(E) Confocal imaging of FISH (cmyb)/immunofluorescence (PH3) at 60 hpf in CHT of control embryos and ccl25b morphants. White arrowheads mark double-positive cells. Scale bar, 50 μm.

(F) The statistical data showing that the percentage of double-positive/cmyb⁺ cells was reduced significantly in the morphants (mean ± SD, t test; ^∗∗∗p < 0.001, n = 8).

(G) qPCR result of cell division-related gene expression in control and ccl25b morphants (mean ± SD, t test; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ns > 0.05, n = 3).

See also Figure S6.