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Fig. 2
pgrn-a-targeted morpholino oligonucleotides inhibit Pgrn-a translation, which results in microphthalmia. (A) Western blot showing Pgrn-a and Actin expression in 72 hpf uninjected (UI) and morphant (5′UTR MO) embryos. (B) Histogram showing quantification of Pgrn-a expression in UI controls (26.2 ± 2.05%) and morphants (2.7 ± 0.9%); **p ≤ 0.01. Quantitative data from three biological and technical replicates is normalized to Actin and represented as mean; error bars represent the standard deviation. (C–H) Representative whole embryos from control (C–F) and experimental (G–H) groups at 72 hpf. 5′UTR MM MO and SS MM MO, embryos injected with 5-nucleotide mismatch control morpholinos; 5′UTR MO, embryos injected with pgrn-a 5′UTR-targeted morpholinos; SS MO, embryos injected with pgrn-a e3i3 splice site-targeted morpholinos.