Fig. S2
miR-9 expression in NSCs in the zebrafish brain (related to Figure 1)
(A, B) Confocal section of double in situ/immunolabelling showing extensive overlap in the expression of endogenous miR-9 and EGFP protein in hindbrain at 72 hpf or in the ventricular zone of the adult zebrafish telencephalon in the Tg(gfap:gal4), Tg(uas:egfp) line, revealing miR-9 expression in embryonic and adult NSCs. (C) Whole-mount in situ hybridization against miR-9 showing the time course of miR-9 expression in the developing embryo at 24, 36 and 48 hpf. Dorsal view of the brain with anterior up. Lateral view of the retina. Scale bars: 10 μm (A, B) or 100 μm (C).