Fig. 6
Alx1 is a novel target of zic2 in the periocular neural crest. Embryos derived from zic2agbt133/+; zic2bt104/zic2bt104 parents were stained for alx1 expression by WISH. A: normal expression in periocular mesenchyme of sibling embryo. B: depleted expression in zic2 (arrow) in mutant embryo (39 out of 112 total, 2 expts). C-G: wild type embryos stained for alx1 expression by WISH. C-D: alx1 is expressed in frontonasal neural crest at 16 hpf. E, F: alx1 is expressed in periocular mesenchyme (*) at 24 hpf and 36 hpf. G: alx1 is expressed in the ethmoid plate (arrowhead) at 48 hpf. Embryos in A, B, C, and E are shown in lateral views, anterior to the left. Embryo in D is shown dorsally, anterior to the left. Embryos in F and G are shown in anterior views, ventral at the top.
Reprinted from Developmental Biology, 429(1), Sedykh, I., Yoon, B., Roberson, L., Moskvin, O., Dewey, C.N., Grinblat, Y., Zebrafish zic2 controls formation of periocular neural crest and choroid fissure morphogenesis, 92-104, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.